Targesphere™ Acoustic Transfection Agent

SV-LEC Targesphere GFP
  • SV-LEC Targesphere GFP

    SV-LEC cells 24h after treatment with Targesphere and a GFP-coding plasmid (courtesy M Lawrence, U.Va)

  • Targesphere and Renal Mesangial Cells siRNA YoYo1

    Renal mesangial cells showing intracellular delivery of fluorescently labelled siRNA (Courtesy K. Sharma, UCSD)

  • HEK293 with Targesphere and GFP

    293 cells 24h after treatment with Targesphere and a GFP-coding plasmid (courtesy M Lawrence, U.Va)

  • Targesphere with Plasmid using ToTo3

    Targesphere agents loaded with plasmid DNA stained with YoYo-1

Applications

Targesphere is a cationic delivery agent specifically engineered to be used for in vivo and in vitro transfection of plasmids for over expression or fluorescence tagging, and also introduction of siRNA, shRNA or antisense. Transfection agents can be coupled to the surface of Targesphere by simple incubation techniques and activated by ultrasound energy for intracellular delivery. Optimal dosages vary with application.

Applications PDF: 
application/pdf iconTargeted Gene Therapy by Sonoporation in an Experiemental Crohn's Disease Model

Directions for Use

Targesphere consists of a dispersion of cationic, lipid/polymer microspheres encapsulating a core of insoluble gas. Plasmid, siRNA and other anionic payloads can be coupled to the surface of the Targesphere agents by simple incubation. Intracellular delivery can be achieved by activation of the Targesphere agents with acoustic energy.

Specifications

Targesphere is a cationic delivery agent used for transfection in vitro and in vivo. Plasmid, siRNA and other anionic payloads can be coupled to the surface of the Targesphere agents by simple incubation. Intracellular delivery is mediated by activation of the Targesphere agents with acoustic energy. Optimal dosages vary with application. Please contact Targeson technical support for protocol assistance.